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~Welcome~

A true love of all things natural and beneficial to the body, mind, and spirit. Intrigued with knowledge regarding herbs and foods for better health.  The focus here is improve the way we eat, think, and feel.  I love juicing, raw foods, growing my own food, and re-purposing, recycling and re-designing old garden, farm, home, and yard items for all kinds of uses and decor in and around the home and garden…in other words – I have a blast and love what I do.

We have a little studio/shop where we will soon begin to turn trash into treasures… planters, lights, birdhouses, planting boxes, infinite possibilities.  I am so excited about this I can barely sleep.  We will also build some benches and tables and restore some wonderful old things.  Everything from our heart and into the house, porch or yard.  Yay!!!

The Greenhouse is in the planning stage and hopefully here by Spring, which is only a few weeks away!!  There will be herbs and veggies and plants.  The Greenhouse will be about 8′ x 20′ so we will see just how much I can cram in it but it should be awesome.  And then there is composting…not something I am familiar with at all but I am ready to tackle it for the sake of the soil that will produce the edible garden and the beauty of the plants.

We also have a beautiful guest lodging… Dragonfly Mountain Lodge
It is my opinion the brain functions better with a healthier body.  I could be wrong …. probably not. :)

Piperlongumine was a direct STAT3 inhibitor and caused regression of breast cancer cell line xenografts in nude mice.

PMID:

Oncogene. 2014 Mar 31 ;0. Epub 2014 Mar 31. PMID: 24681959

Abstract Title:

Drug-repositioning screening identified piperlongumine as a direct STAT3 inhibitor with potent activity against breast cancer.

Abstract:

Signal transducer and activator of transcription (STAT) 3 regulates many cardinal features of cancer including cancer cell growth, apoptosis resistance, DNA damage response, metastasis, immune escape, tumor angiogenesis, the Warburg effect and oncogene addiction and has been validated as a drug target for cancer therapy. Several strategies have been used to identify agents that target Stat3 in breast cancer but none has yet entered into clinical use. We used a high-throughput fluorescence microscopy search strategy to identify compounds in a drug-repositioning library (Prestwick library) that block ligand-induced nuclear translocation of Stat3 and identified piperlongumine (PL), a natural product isolated from the fruit of the pepper Piper longum. PL inhibited Stat3 nuclear translocation, inhibited ligand-induced and constitutive Stat3 phosphorylation, and modulated expression of multiple Stat3-regulated genes. Surface plasmon resonance assay revealed that PL directly inhibited binding of Stat3 to its phosphotyrosyl peptide ligand. Phosphoprotein antibody array analysis revealed that PL does not modulate kinases known to activate Stat3 such as Janus kinases, Src kinase family members or receptor tyrosine kinases. PL inhibited anchorage-independent and anchorage-dependent growth of multiple breast cancer cell lines having increased pStat3 or total Stat3, and induced apoptosis. PL also inhibited mammosphere formation by tumor cells from patient-derived xenografts. PL’s antitumorigenic function was causally linked to its Stat3-inhibitory effect. PL was non-toxic in mice up to a dose of 30 mg/kg/day for 14 days and caused regression of breast cancer cell line xenografts in nude mice. Thus, PL represents apromising new agent for rapid entry into the clinic for use in treating breast cancer, as well as other cancers in which Stat3 has a role.Oncogene advance online publication, 31 March 2014; doi:10.1038/onc.2014.72.

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α-mangostin suppressed the proliferation, migration, and invasion of pancreatic cancer cells.

PMID:

Biomed Res Int. 2014 ;2014:546353. Epub 2014 Apr 10. PMID: 24812621

Abstract Title:

α-Mangostin suppresses the viability and epithelial-mesenchymal transition of pancreatic cancer cells by downregulating the PI3K/Akt pathway.

Abstract:

α -Mangostin, a natural product isolated from the pericarp of the mangosteen fruit, has been shown to inhibit the growth of tumor cells in various types of cancers. However, the underlying molecular mechanisms are largely unclear. Here, we report that α -mangostin suppressed the viability and epithelial-mesenchymal transition (EMT) of pancreatic cancer cells through inhibition of the PI3K/Akt pathway. Treatment of pancreatic cancer BxPc-3 and Panc-1 cells with α -mangostin resulted in loss of cell viability, accompanied by enhanced cell apoptosis, cell cycle arrest at G1 phase, and decreaseof cyclin-D1. Moreover, Transwell and Matrigel invasion assays showed that α -mangostin significantly reduced the migration and invasion of pancreatic cancer cells. Consistent with these results, α -mangostin decreased the expression of MMP-2, MMP-9, N-cadherin, and vimentin and increased the expression of E-cadherin. Furthermore, we found that α -mangostin suppressed the activity of the PI3K/Akt pathway in pancreatic cancer cells as demonstrated by the reduction of the Akt phosphorylation by α -mangostin. Finally, α -mangostin significantly inhibited the growth of BxPc-3 tumor mouse xenografts. Our results suggest that α -mangostin may be potentially used as a novel adjuvant therapy or complementary alternative medicine for the management of pancreatic cancers.

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